Stability-indicating liquid chromatographic method for quantification of new anti-epileptic drug lacosamide in bulk and pharmaceutical formulation
2012
An isocratic stability indicating reversed-phase liquid chromatographic
determination was developed for the quantitative determination of lacosamide
in the pharmaceutical dosage form. A Hypersil C-18, 4.5μm column with mobile
phase containing acetonitrile-water (20:80, v/v) was used. The flow rate was
1.0 mL min-1 and effluents were monitored at 258 nm. The retention time of
lacosamide was 8.9 min. The method was found to be linear in the
concentration range of 5-100 μg/ml and the recovery was found to be in the
range of 99.15 - 100.09 %. The limit of detection and limit of
quantification were found to be 2 μg/ml and 5 μg/ml, respectively.
Lacosamide stock solutions were subjected to acid and alkali hydrolysis,
chemical oxidation and dry heat degradation. The drug was found to be stable
to the dry heat and acidic condition attempted. The proposed method was
validated and successfully applied to the estimation of lacosamide in tablet
dosage forms.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
8
Citations
NaN
KQI