Bifunctional dialkyl disulfide reagent having terminal succinimidoxy ester group. Fabrication of gold surfaces with bioaffinity ligands for impedimetric biosensors

A gold electrode surface was modified with the self-assembled monolayer of bis(10-(N-succinimidoxy) carbonydecyl) disulfide (1). Electrochemical properties of the electrode were characterized by cyclic voltammetry and impedance measurements. The former technique showed a surface coverage ratio of 0.34 was attained. By taking advantages of the reaction of the terminal succinimidoxy ester group of 1 and amino groups, the electrode was applied to enzyme immobilization. A possible application to an electrochemical sensor of the resulting modified electrode based on an impedimetric principle was also investigated.