Partial Purification of Cellulase from Clostridium thermocellum

Summary: The cellulolytic system from culture filtrates of Clostridium thermocellum, when partially purified by preparative electrophoresis, showed a single protein band on analytical polyacrylamide gel electrophoresis; however, sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that at least five proteins were present. Carbohydrate was present in the purified fraction and it is suggested that the cellulase was present as a complex.