Biological effects of high-voltage prickle electrostatic fields on mouse Lewis lung cancer cells in vitro

Objective To investigate the effects of high-voltage prickle electrostatic fields (HVPEF) on proliferation and drug susceptibility of mouse Lewis lung cancer cells. Methods MTT method was adopted to detect A values of cells for deducing relative activity of cells in group after HVPEF, group treated only with carboplatin, group treated with both HVPEF and carboplatin. Results In group after HVPEF, the cell growth was stimulated with voltages ≤1.5 kV. At voltage 1.5 kV, the relative activity of cells was 132.7% compared with control group (P0.05); the cell growth was inhibited after voltages 1.5 kV, the best inhibitory voltage was 2.5 kV and the relative activity of cells in 2.5 kV group merely was 71.7% of that in control group (P0.05); Moreover, the effects of HVPEF and carboplatin together on the cells were significant, the relative activity of cells in group treated with both HVPEF and carboplatin was 57.8% of that in control group (P0.01). Conclusion Different intensities of HVPEF can directly inhibit the growth of tumor cells and increase the susceptibilities of tumor cells to chemotherapy drugs.