Flavonoids from the seeds of Oroxylum indicum and their anti-inflammatory and cytotoxic activities

Abstract Two new flavonoid glycosides, named oroxin C ( 1 ) and oroxin D (scutellarein 4'-methyl ether 7- O - β -D-glucopyranosyl-(1→6)- β -D-glucopyranoside, 2 ), together with eight known flavonoids ( 3 – 10 ), were isolated from the seeds of Oroxylum indicum . Their structures were elucidated unambiguously by spectroscopic techniques and chemical methods. Anti-inflammatory and cytotoxic activities of all the isolated compounds were evaluated. Chrysin ( 3 ) and chrysin-6-C- β -D-glucopyranosyl-8-C- α -L-arabinopyranoside ( 10 ) showed moderate inhibitory effects aganist the LPS-induced NO production in murine RAW264.7 macrophages with IC 50 values of 18.63 ± 0.91 and 28.69 ± 0.43 μ M, respectively. Chrysin ( 3 ) exhibited weak cytotoxic activity aganist A549, HepG2 and SW480 human cancer cell lines with IC 50 values of 40.88 ± 3.85, 50.55 ± 2.59 and 91.60 ± 4.27 μ M, respectively.