Preparation of multivalent egg yolk IgY antibodies against Helicobacter pylori

: Objective To prepare a specific multivalent immunoglobulin of yolk (IgY) against multi-virulence factors of Helicobacter pylori (Hp). Methods The purified recombinant BabA2, UerB and FlaA of Hp were mixed equally with adjuvant, and then used to immunize egg-laying hens to produce specific multivalent IgY. Next, the biological characteristics of purified IgY were analyzed by SDS-PAGE, Western blot and ELISA. To analyze the inhibition effects of multivalent IgY on the growth of Hp in vitro, Hp was co-cultured with different final concentration of IgY (1, 5, 10) mg/mL in the presence or absence of antibiotics. Results After immunization, the titer of egg yolk IgY reached up to 1:150 000. Furthermore, the bacteriostasis assay showed that the multivalent IgY had a significant inhibitory effect on the growth of Hp at 5 mg/mL, and the combination with amoxicillin could further inhibited the growth of Hp. Conclusion The specific multivalent IgY against Hp is successfully prepared.