ASSESSMENT OF SPASMOLYTIC ACTIVITY OF ALCOHOLIC EXTRACT OF ACILLEA MILL EFOLIUM , RUBIA CORDIFOLIA AND SAUSSUREA LAPPA IN WISTAR ALBINO RATS

The present study was carried out to e valuate the potential of alcoholic extract of anti - inflammatory plants viz. Achillea millefolium , Rubia cordifolia and Saussurea la ppa for spasmolytic activity in rat model using radnoti organ bath . The relaxant effect of all extracts on pre - contracted rat tracheal chain by carbachol (30 µM) had been screened. Isometric contractions of isolated rat tracheas were recorded at 1.4 g rest ing tension and carbachol dose - response curves were performed. EC 50 values (27.12, 13.13 and 7.32 µM, respectively) were identified by plotting cumulative concentration response curve and pD 2 values (4.48 ± 0.99, 6.06 ± 1.03 and 7.41 ± 0.97, respectively) were calculated for individual alcoholic extract of plant . All extracts were able to relax carbachol pre contracted tracheas significantly in a concentration dependent manner. Our results suggested potential role of Achillea millefolium , Rubia cordifolia a nd Saussurea lappa in asthma for further potential therapeutic and clinical uses.