Polymorphism Analysis of MSTN Gene Digested with DraI of Guizhou Black Goat and Qianbei Ma Goat

Black Goat and Qianbei Ma Goat were taken as materials in this study,a primer was designed based on goats MSTN genetic sequences(GenBank registration number: EF588035),and PCR-RFLP method was used to analyze the sequences polymorphism.The results showed that the TTTTA insert existed in 207 bp,led to the emergence of a DraI enzyme polymorphism site.Homozygous wild type of black goat was advantage genotype while heterozygous type(AB)and homozygous mutant type(BB) were disadvantage genotypes.A gene was a protogene;Meanwhile homozygous wild type of Qianbei Ma Goat(CC) was advantage genotype while heterozygous type(CD)and homozygous mutant type(DD) were disadvantage genotypes and C gene was a protogene.