Synthesis and erythropoietin receptor binding affinities of N, N-disubstituted amino acids

Abstract N,N- Dicinnamyl, N -benzyl- N -cinnamyl, and N,N- dibenzyl amino acids were prepared and evaluated in an EPO binding assay. Several derivatives of aspartic acid, glutamic acid, and lysine exhibited moderate (10–50 μM) affinity for EBP; ‘dimerization’ of the most potent analogues by coupling with linear diamines led to EPO competitors having 1–2 μM binding affinities.