Determination of Aloin Content in Aloe with HPLC and Analysis of Aloe Chromatogram

[Objective] To establish a HPLC method for determining the content of aloin in 5 species of Aloe, and analyze their chromatograms, so as to distinguish them from each other. [Method] The content of aloin in sample solutions of Aloe species prepared using ultrasonic wave method was measured by HPLC. And, the chromatograms of Aloe were obtained. [Result] The linear regression equation of aloin was Y=13.1684X-2.8554, R=0.9998, showing good linearity between concentration and peak area in the range of 2.92 to 46.67μg/ml. The average recovery rate was 108.0%. The content of aloin was 614.3μg/g for A. arborescens, 212.5μg/g for A. delaetii, 135.3μg/g for A. vera L., 72.92μg/g for A. vera L. var. chinesis (Haw.) Berg, and 2.62μg/g for A. nobilis. Double peaks occurred at the retention time of 3 to 5min in the HPLC chromatogram of A. vera L., the peak height of which was slightly higher on the left, but this difference was not significant. Its double peaks were basically separated at the baseline. From then on, no obvious chromatographic peaks appeared on the chromatogram. In addition, the chromatogram of A. vera L. was similar to that of A. arborescens and very different from that of other three species. [Conclusion] There is a clear difference in aloin level among 5 species of Aloe. Of these, the content of aloin in A. arborescens is the highest. The determination method is stable, accurate and quick. Five species of Aloe are distinguished from each other by means of chromatogram analysis.