Safety Assessment and Bioefficacy of Fluopyram 20 %+Tebuconazole 20 %-40 SC in Chilli, Capsicum annum L. Against Anthracnose Disease

The present paper reports the in vitro and in vivo bioefficacy of different doses of a combination of fungicide, fluopyram 20 %+tebuconazole 20 %-40 SC against anthracnose disease of chilli (Capsicum annuum L.) and their pre-harvest intervals pertaining to two different application rates. In vitro bioefficacy studies revealed the best control (79.3 %) at 250 mg kg−1 against 68.4 % control under field conditions. A sample preparation method based on ethyl acetate extraction and estimation by LC–MS multiple reaction monitoring was validated in chilli fruits. Residue dissipation studies were conducted in field at single and double doses. The residues of both fluopyram and tebuconazole on all the sampling days were below the maximum residue limit (European Union) and maximum permissible intake estimated on the basis of the prescribed acceptable daily intakes. The combined bioefficacy and residue dynamics information will support label-claim of this combination of fungicide for the management of anthracnose in chilli and facilitate its inclusion in good agricultural package of practices.