Bud Induction and Fast Propagation in Emblic

The tender bud and shoot segment of emblic were in vitro cultured on the MS medium. Results indicated that the addition of BA had a marked promoting effect on bud induction. The optimal hormone combination was found to be BA with NAA. For the bud explant, the optimal medium dosage for bud induction was MS added with 0.5 mg/L of BA and 0.1 mg/L of NAA, and the differentiating rate was above 90%. And for the shoot explant, the concentration of BA required for bud induction was higher, and the optimal dosage was MS added with 2.0 mg/L of BA and 0.1 mg/L of NAA, the differentiating rate was 56.7%. For bud multiplication, the medium used was MS added with 0.1 mg/L of BA, 0.1 mg/L of NAA, and 50 mg/L of NaH 2PO 4. For root induction, the medium used was 1/2 MS added with 0.25 mg/L of NAA and 0.25 mg/L of IBA, which generated a rooting rate of 30%.