Synthesis, antioxidant and antimicrobial activity of carbohydrazones

Fourteen mono - and bis -carbohydrazone ligands have been synthesized and characterized. Antioxidant activity of the substances was investigated together with possible ( E ) / ( Z ) isomerization, and explained on the most active antioxidant compound a4 in various dimethylsulphoxide–water mixtures. The addition of water to the system was involved in the formation of hydrated molecules which was confirmed in NMR after the addition of D 2 O. The ligands were tested in vitro against Gram-positive and Gram-negative bacteria and fungis, and their activity was discussed in relation to the structure of investigated carbohydrazone.