Establishment of molecular identity for kenaf germplasm using ISSR and RAPD markers.

【Objective】 Fifty-one accessions of kenaf germplasm from different countries and areas,which contain cultivated,wild and related species,were genetically analyzed in order to establish molecular identity of kenaf germplasm.【Method】 Various types of kenaf were used for inter-simple sequence repeat(ISSR) and random amplified polymorphic DNA(RAPD) analysis.The genetic similarity coefficients were estimated and cluster analysis were carried out using UPGMA method.【Result】 Nineteen ISSR primers generated 113 bands,of which 101 were polymorphic and PPB was 89.38%.Twenty RAPD primers generated 118 bands,of which 112 were polymorphic and PPB was 94.92%.The results showed that diversity of kenaf gerplasm was high.Fifty-one specific molecular identities could be established by three methods,which were specific bands,typical band patterns and primer combinations.【Conclusion】 The higher genetic diversity revealed higher genetic distance and wider genetic base.ISSR and RAPD can be used for establishing molecular identity of kenaf germlasm.