Flavonoids from Diospyros kaki Inhibit the Adhesion between Lymphocyte and Dorsal Root Ganglion

Objective:To investigate the effects of flavonoids from the leaves of Diospyros kaki L (FLDK) on the adhesion between the lymphocyte and the neurone. Methods:Centrifugal assay for fluorescence-bsaed cell adhesion was used to assay the adhesion between the lymphocyte and the dorsal root ganglion (DRG). Results:The adhesion was significantly suppressed in the presence of FLDK dose-dependently at 5,25 μg/mL concentration. FLDK was also effective to inhibit the adhesion under the challenge of ICAM-1 by 28.5% and 50.1%,respectively. Furthermore,FLDK enforced the inhibition of anti-NCAM antibody on the lymphocyte adhesion to DRG cells. Conclusion:FLDK might contribute to the prevention and treatment of the inflammation injury under neuron insult such as ischemia/reperfusion,neurotrauma and other neurodegenerative disease by inhibiting the adhesion between lymphocytes and neurons.