Antibacterial Compounds from Mushrooms II: Lanostane Triterpenoids and an Ergostane Steroid with Activity Against Bacillus cereus Isolated from Fomitopsis pinicola

Anti− BACILLUS CEREUS bioassay-guided fractionation of a crude extract of the American mushroom, FOMITOPSIS PINICOLA, was performed using thin-layer chromatography, Sephadex LH-20 column chromatography, and preparative-scale HPLC. Five lanostane triterpenoids ( 1- 5) and one ergostane steroid ( 6) were isolated and identified. Compound 1 is a new lanostane triterpenoid, and its structure was determined using 1D and 2D NMR experiments, HR-MS, and physical data. Each of the purified compounds ( 1- 6) was tested for antibacterial activity against B. CEREUS using standard MIC assays. Compounds 1- 6 had MIC values of 32, 16, 32, 32, 128, and 64 μg/mL, respectively.