Purification and characterization of arylsulfatase from sea urchin (Hemicentrotus pulcherrimus) embryos

Abstract 1. 1. Arylsulfatase was extracted from sea urchin ( Hemicentrotus pulcherrimus ) plutei and purified to electrophoretical homogeneity by means of DEAE-cellulose, acetone fractionation and Sepharose CL-6B, successively. 2. 2. The molecular weight of this enzyme was approx, 670,000. The molecular weight of a single subunit was approx. 63,000. The K m value for p -nitrophenyl sulfate was 0.59 mM. 3. 3. This enzyme was competitively inhibited by the sulfate ion and was classified as the type II arylsulfatase. The pH optimum was between 5.0 and 6.0.