Preparation method of cephalotaxus alkaloid product

The invention belongs to the field of plant endophyte separation and microorganism fermentation engineering, and in particular relates to a preparation method of a cephalotaxus alkaloid product. The preparation method comprises the following steps: by adopting roots, stems and leaves of perennial cephalotaxus as explants, cutting into strips, sterilizing, then culturing in a culture medium to grow cephalotaxus symbiotic bacteria, then separating strains capable of metabolizing cephalotaxus alkaloid from the cephalotaxus symbiotic bacteria and performing culture and fermentation, concentrating a fermentation liquid, and then precipitating to prepare a product. According to the preparation method disclosed by the invention, in a preparation process of the fermentation liquid, a second batch of strains are inoculated after the strains generate metabolic organisms, metabolic products of the former batch of the strains are used for providing nutrient substances for the second batch of the strains, so that the growth of the second batch of the strains can be greatly promoted, and the biological activity of the strains also can be improved, and thus a great number of fermentation liquids can be generated, and the yield of cephalotaxus alkaloid can be improved.