[Inhibition of vegfr-2 gene expression by RNA interference].

This study was aimed to explore a novel potential gene taget for therapy of malignant tumor.The recombinant expression plasmids of VEGF/VEGFR-2 were designed,constructed and then transfected into A549 cells by using lipofectamine.The expressions of VEGF/VEGFR-2 mRNA and protein were detected by RT-PCR and Western blot respectively.The cell proliferation was assayed by CCK-8 and the cell apoptosis was detected using Hoechst Staining.The results indicated that as compared with the blank control,pGenesil-1 and scramble groups,the interference effect of pGenesil-1-vegfr-2-shRNA-1 vector group was more obvious.As the expression of endogenous vegfr-2 mRNA decreased,the expression of VEGFR-2 protein decreased correspondingly.The proliferation of A549 cells was inhibited significantly by RNAi at 72 hours(p0.01).The apoptosis of A549 cells was induced at 48 hours after being transfec-ted with pGenesil-1-vegfr-2-shRNA-1 and the typical apoptosis morphology could be seen by fluorescence microscopy.It is concluded that the expression of vegfr-2 gene is inhibited effectively by vegfr-2 specific shRNA.The proliferation of A549 cells is inhibited significantly and the apoptosis of cells is induced.This result showed that VEGF/VEGFR-2 signaling pathway can be an effective taget for the prevention and treatment of malignant tumor.