Gluten formation from flour of kernels in developing wheat grain

The proteins that form gluten of a winter wheat cultivar, Ukrainka (HMW-GS composition 1, 7+8, 5+10) grown in Hungary and harvested in the year 2006, was investigated during grain development. The formation of gluten, its protein fractions and composition of polymeric fraction, were followed starting at the 12 th day after anthesis (DAA) to the 52 nd .Gluten formation was first observed (manual method of determination) only 20–25 days after anthesis and its quantity increased rapidly during the next period of grain development. The gluten was separated to a fraction extractable with SDS-phosphate buffer and another fraction, unextractable by this buffer. An increase in the ratio of unextractable polymeric protein (UPP)/soluble protein fraction was observed during grain development. As expected, gliadin was the main component of the soluble fraction and glutenin that of the insoluble one. HMW monomers were detected in unreduced fractions in small quantities only until 30 th DAA. RP-HPLC of reduced gluten f...