Long-term Zinc and Iron Supplementation in Children of Short Stature: Effect of Growth and on Trace Element Content in Tissues

Summary We evaluated the effect of one year of supplementation with iron plus zinc (12 mg /day of Fe+++ and 12.5 mg/day of Zn++ ), zinc alone (12.5 mg/day of Zn++) and placebo on growth and on the iron, zinc, copper and selenium tissue contents in 30 well-selected children of short stature (16 M and 14 F; 4–11 years old). Before and after supplementation, we measured the concentrations of iron, transferrin, ferritin, zinc and copper in serum, of zinc in erythrocytes and leukocytes, and of zinc, copper and selenium in hair, as well as glutathione peroxidase activity in erythrocytes. Before supplementation, ferritin and serum, erythrocyte and hair zinc contents were significantly lower than in age-matched controls, while the other measured indices were in the normal range. Iron plus zinc supplementation caused an improvement in growth rate in all subjects, i.e., the median Z-score increased from -2.22±0.45 to -0.64±0.55; (p