Comparison of hypoxic preconditioning and hypoxic postconditioning on expression of heme oxygenase-1 in rat myocardial cells

【Objective】 To compare the effect of hypoxic preconditioning and hypoxic postconditioning on expression of heme oxygenase-1 in rat myocardial cells.【Methods】 The cultured rat cardiomyocytes were randomized into 4 groups: Control group,hypoxic preconditioning group,hypoxic postconditioning group and combination group(hypoxic preconditioning plus hypoxic postconditioning).The levels of LDH and HO-1 were determined respectively in all groups at different time.【Results】 The level of LDH was significantly lower in hypoxic preconditioning group and in hypoxic postconditioning group than in control group(both P 0.01),while the level of HO-1 was significantly higher than in control group(both P 0.05).The levels of LDH and HO-1 in combination group were not differ statistically from hypoxic pre-and postconditioning group(both P 0.05).【Conclusion】 The results indicate that the cardioprotection of hypoxic preconditioning and hypoxic postconditioning on myocardial cells could partly be realized by increasing heme oxygenase-1 in myocardium and probably by activating the same signal transduction pathway.