Technologies for Cryopreservation: Overview and Innovation

In the vitrification process, the use of CryoProtectant Agents and the increasing of cooling rate (from 2,500 /min to 130,000 /min) avoid the ice crystal formation, increasing the embryos and oocytes survival. Unfortunately, common cryoprotectants are toxic and the immersion of solution directly in liquid nitrogen can be cause of contamination of embryos and oocytes with bacterium, mushroom and virus.