Antioxidant activity comparison between non-polysaccharides and crude polysaccharides from Dendrobium officinale stems in vitro and in vivo

Objective: To compare the antioxidant activities of non-polysaccharides and crude polysaccharides from Dendrobium officinale stems in vitro and in vivo. Methods: Crude polysaccharides from Dendrobium stems was extracted with water and then continuously deposited by ethanol,non-polysaccharides was extracted with 85% ethanol. Their antioxidant activities were evaluated by various systems in vitro,including scavenging activity of superoxide anion,ABTS and DPPH radicals with Vc as positive control. Feed the rats with high glucose and high fat diet,and drink alcohol. Total extract,non-polysaccharides and crude polysaccharides( dosages were 1,2 g / kg) were administered for 6 weeks,then measured T-AOC,SOD,MDA on serum. Results: Both samples showed inhibitory effects on superoxide,ABTS and DPPH radical( IC50 of non-polysaccharide was 3. 535,0. 357 and 1. 545 mg / ml while 4. 916,6. 597 and 2. 077 mg / ml with polysaccharides). All of extractives could increase T-AOC and SOD( P < 0. 01,P < 0. 05),reduce MDA( P < 0. 05). Conclusion: Non-polysaccharide showed strong antioxidant activity in vitro and in vivo. In ABTS,non-polysaccharide showed stronger antioxidant activity,which worth further developing and utilizing.