A kind of method simultaneously detecting multiple liposoluble vitamin in blood sample

The invention discloses a kind of method simultaneously detecting multiple liposoluble vitamin in blood sample, first by simple liquid-liquid extraction method, pre-treatment is carried out to biological specimen, then chromatographic resolution and Mass Spectrometer Method is entered, a pair qualitative ion and a pair quota ion is selected respectively for often kind of vitamin, using the relative retention time of various vitamin and qualitative ion pair as qualitative foundation, quantitative with standard items production standard curve.Meanwhile, this method applies accuracy, the validity of the quality-control product investigation method of three levels, avoids testing result distortion.The present invention achieves the object that application LC-MS technology detects kind of the liposoluble vitamin of six in a serum sample simultaneously first, reduce chaff interference impact, the method fast analysis time easy and simple to handle only 4.8min, high flux, cost are low, liposoluble vitamin level in effective monitoring human, reasonable, the safety of vitamin are supplemented there is directive significance, be easy to clinical expansion and popularize.