MicroRNA expression profiling using LNA-modified probes in a liquid-phase bead-based array

Here we describe a new liquid-phase, bead-based array for in-solution expression analysis of microRNAs (miRNAs). The array combines Locked Nucleic Acid (LNA™)-modified capture probes and the xMAP® multiplexing bead technology. Incorporation of LNA in the array capture probes greatly increases their affinity for their short miRNA targets, thereby adding selectivity to the array. As a result, the FlexmiR™ system offers a highly specific, robust and fast miRNA profiling platform.