Content Determination of Trace Elements in Euphrasia regelii by Flame Atomic Absorption Spectrometry

OBJECTIVE:To determine the content of trace elements in Euphrasia regelii.METHODS:A concentrated nitric acid and perchloric acid(4:1,V:V) mixture solution was used to digest the herbal sample,and flame atomic absorption spectrometric(FAAS) was applied to determine the content of trace elements(Fe,Cu,Zn,Mn).RESULTS:The contents of trace elements(Fe,Cu,Zn,Mn) in E.regelii were 739.84 μg·g-1,16.04 μg·g-1,108.86 μg·g-1 and 49.20 μg·g-1,respectively.Average recoveries were 97.6%～103.0%,and RSD ranged 0.8%～3.5%(n=6).CONCLUSION:E.regelii contains rich trace elements(Fe,Cu,Zn,Mn).The method is simple and accurate,and trial result can provide reference for the further study of pharmacological action of E.regelii.