Inhibitory effects of 2,3,5,4′-tetrahydroxystilbene-2-O-β-d-glucoside on experimental inflammation and cyclooxygenase 2 activity

The inhibitory effects of 2,3,5,4′-tetrahydroxystilbene-2-O-β-d-glucoside (THSG), extracted from the roots of Polygonum multiflorum Thunb, on inflammatory activity in animal models and cyclooxygenase-2 (COX-2) activity in lipopolysaccharide (LPS)-induced mouse RAW264.7 macrophage cells were investigated. The carrageenin (CGN)-induced rat paw oedema model and dimethylbenzene-induced mouse ear oedema model were prepared; MTT assay, semi-quantitative RT-PCR, Western blot and ELISA were adopted. THSG 2.3, 4.6 and 9.2 mg kg− 1 by oral administration inhibited mouse ear oedema and the percentage of inhibition of THSG 9.2 mg kg− 1 is 87%. THSG 3.2, 6.4 and 12.8 mg kg− 1 by oral administration dose-dependently inhibited rat paw oedema and the percentage of inhibition of THSG 12.8 mg kg− 1 is 56% at 6 h. Indomethacin 13 and 9 mg kg− 1 showed 90% and 57% inhibition in the same animal models, respectively. LPS 1 μg ml− 1 significantly up-regulated prostaglandin E2 (PGE2) production (inducing COX-2 activity) by 35% (...