Miniaturized hollow fiber assisted liquid-phase microextraction and gas chromatography-mass spectrometry for determination of benzophenone and derivates in human urine sample.

Abstract The determination of benzophenones (BPs) in human urine sample by miniaturized hollow fiber assisted liquid-phase microextraction (HF-LPME) and gas chromatography–mass spectrometry (GC–MS) is described. As analytes, BP, its metabolites benzhydrol (BP-OH) and 2-hydroxybenzophenone (2OH-BP), and its derivatives 2-hydroxy-4-methoxybenzophenone (BP-3) and 2-hydroxy-4-methoxy-4′-methylbenzophenone (BP-10) were selected. The detection limit and the quantification limit of BPs in human urine sample are 5–10 and 20–50 pg mL −1 , respectively. The calibration curve for BPs is linear with correlation coefficient higher than 0.99 in the range of 0.02–10 or 0.05–10 ng mL −1 . The average recoveries of BPs in human urine samples spiked with 0.5 and 5 ng mL −1 BPs are 89.8–100.2% (RSD: 2.5–9.3%) and 89.3–99.9% (RSD: 2.9–3.7%), respectively. Ten human urine samples were analyzed using the present method. BP-OH and BP-3 were detected in all the samples within the range of 0.24–5.91 and 0.43–5.17 ng mL −1 , respectively. This simple, sensitive, and selective analytical method was successfully applied to the determination of trace amounts of BPs in human urine samples.