CCCTC-binding factor locks premature IgH germline transcription and restrains class switch recombination
2017
In response to antigenic stimulation B cells undergo Class Switch Recombination (CSR) at the immunoglobulin heavy chain (IgH) to replace the primary IgM/IgD isotypes by IgG, IgE or IgA. CSR is initiated by activation-induced cytidine deaminase (AID) through the deamination of cytosine residues at the switch (S) regions of IgH. B cell stimulation promotes germline transcription (GLT) of specific S regions, a necessary event prior to CSR because it facilitates AID access to S regions. Here we show that CCCTC-binding factor (CTCF) deficient mice are severely impaired in the generation of germinal center B cells and plasma cells after immunization in vivo, most likely due to impaired cell survival. Importantly, we find that CTCF deficient B cells have an increased rate of CSR under a variety of stimulation conditions in vitro. This effect is not secondary to altered cell proliferation or AID expression in CTCF deficient cells. Instead, we find that CTCF deficient B cells harbour an increased mutation frequency at switch regions, probably reflecting an increased accessibility of AID to IgH in the absence of CTCF. Moreover, CTCF deficiency triggers premature GLT of S regions in naive B cells. Our results indicate that CTCF restricts CSR by enforcing GLT silencing and limiting AID access to IgH.
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