Stable Expression by Lentiviral Transduction of Cells

: Lentiviral gene transfer represents a versatile and powerful method for genetic transduction of many cell lines and primary cells including "hard-to-transfect" cells. As a consequence of the integration of the recombinant lentiviral vector into the cellular genome the transgene is stably maintained and long term producing cells are established. Here, we describe the current state of the art and give details for lab scale production of lentiviral vectors as well as for infection and titration of the viral vectors.