Separation of Amyloid Proteins by Size-Exclusion Chromatography in Aqueous Organic Mobile Phase

Abstract Separation of amyloid proteins was performed by size exclusion chromatography using acidic aqueous 20% acetonitrile, as a volatile mobile phase. Pure major amyloid constituents (proteins AA and AL) were isolated on a Fractogel TSK HW-55 and a Bio-Sil TSK-125 columns. The isolation procedure used is simpler and more rapid comparing with the traditional separation of amyloid proteins (size -exclusion chromatography on Sephadex type columns in 5M guanidine - HCl). The technique described allows to avoid the use of strong denaturants and may be applied for HPLC separation of amyloid proteins on a microgram scale.