Solid-state NMR studies of peripherally membrane-associated proteins: dealing with dynamics, disorder and dilute conditions

This chapter reviews applications of solid-state NMR to three peripheral membrane proteins: cardiolipin-bound cytochrome c, phosphatidylinositol-binding Pleckstrin homology domain and membrane-associated myelin basic protein. We describe solutions to common challenges, such as the dynamics of the membrane-bound protein and the preparation of suitable samples with biological relevance. Modern NMR hardware, molecular biology and sample preparation methods pave the way for effective studies of many interesting peripheral membrane proteins by solid-state NMR.