An efficient protocol for micropropagation of old cypress of Abarkuh (Cupressus sempervirens var. horizontalis [Mill.]) under in vitro condition

An efficient protocol was developed to propagate old cypress of Abarkuh under in vitro condition. The explants from upper part of secondary shoots were successfully proliferated in the woody plant medium (WPM) supplemented with 30 g l−1 sucrose, 7.5 g l−1 agar, 0.5 mg l−1 benzyladenine (BA) and 0.01 mg l−1 indolebutyric acid (IBA). The in vitro raised micro shoots were then elongated by culturing in the WPM supplemented with 0.1 mg l−1 BA. Root induction was successfully conducted by pulse treatment (30 s) in high concentration of IBA (5 g l−1) and subsequent culturing in the WPM supplemented with 2 mg l−1 kinetin (Kin) and 0.01 mg l−1 IBA.