Effect of Trichostatin A on Cell Cycle and the Expression of Cell Cycle-Associated Genes in Human Colon Cancer Cell Lines

To investigate the effects of the histone deacetylase inhibitor,trichostain A(TSA),on cell cycle and apoptosis in human colon cancer cell lines.The colon cancer cell lines (SW480) was treated with the histone deacetylase (HDAC) inhibitor,trichostatin A (TSA) and the cell cycle distribution was studied by flow cy-tometry (FCM).The expression of p21Waf1/Cip1,p27Kip1,CDK2,CyclinE and CyclinA were detected by west-ern-blot.The results indicated that TSA delayed cell cycle G1-S progression and inhibited accumulating of cyclinE,cyclinA.No obvious changes were observed in apoptosis after TSA treatment.The expression of p21Waf1/Cip1?p27Kip1 was markly increased and the expression of CDK2,CyclinE,CyclinA was down-regulated after treated with TSA.It could be concluded that in human colon cancer cell line,HDAC inhibitor,TSA in-duced a G1 cell cycle arrest and have effect on cell proliferation through stimulating the p21Waf1/Cip1,p27Kip1 gene expression and blocking the CDK2,CyclinE,CyclinA expression.Theoretic proof was provided for clinical treatment by histone deacetylase inhibitor in human colon cancer.