Light-sheet fluorescence microscopy with metalens

We propose a Light-sheet fluorescent microscopy (LSFM) system with a flat metalens, which is used to replace the bulky illuminating components. The metalens is a diffractive optics elements (DOEs) composed of various gallium nitride (GaN) nanopillar for light-sheet generation, and it can be readily integrated in existing LSFM systems. In contrast to the traditional LSFM system, the metalens-based system is more compact and flexible, and its sectioning capability is comparable to the conventional illumination arm in LSFMs. The live fluorescent-labeled transgenic Caenorhabditis elegans (C. elegans) are exploited to verify the performance of this system. The intensity profile of oocytes in C. elegans with cellular resolution level was obtained.