Mitigating negative impacts of heat stress in growing rabbits via dietary prodigiosin supplementation

Abstract The adverse influences of elevated ambient temperatures during the summer season on the rabbit industry have received increased global attention. Prodigiosin (PRG) is a natural pigment with powerful antioxidant activities. In this study, the aim was to explore the potential action of PRG for mitigating the negative impacts of heat stress in growing rabbits. A 120 growing rabbits (weaned, males, 35 days of age) were randomly allocated into four treatments of 30 rabbits each. The first group was received a basal diet without PRG (0 g/kg diet), and the 2, 3, and 4 groups received diets including PRG (50, 100, 150 mg/kg diet, respectively) for eight consecutive weeks during the summer season. Results showed that PRG improved significantly final body weight (FBW), daily body weight gain (DBWG), and feed conversion ratio (FCR), but did not affect feed intake (FI). Compared with the control, PRG addition with 50, 100, and 150 mg/kg diet decreased significantly total bilirubin (TB), triglycerides (TG), gamma-glutamyl transferase (GGT) and creatinine levels. The plasma levels of total antioxidant capacity (TAC) and glutathione in the group received (100mg/kg) diet were significantly higher than those in the other groups. Still, MDA was markedly reduced in PRG-treated groups compared with the control. Pro-inflammatory cytokines levels (interleukin-4 (IL-4) and Interferon gamma (IFNγ) were significantly decreased (P