Antibiotics potentiating potential of catharanthine against superbug Pseudomonas aeruginosa

Abstract Multidrug resistance (MDR) put an alarming situation like preantibiotic era which compels us to invigorate the basic science of anti-infective chemotherapy. Hence the drug resistant genes/proteins were explored as promising drug targets. Keeping this thing in mind, proteome of Pseudomonas aeruginosa PA01 was explored, which resulted in the identification of tripartite protein complexes (MexA, MexB and OprM) as promising drug target for the screening of natural and synthetic inhibitors. The purpose of present investigation was to explore the drug resistance reversal potential mechanism of catharanthine isolated from the leaves of Catharanthus roseous. Hence, the test compound catharanthine was in-silico screened using docking studies against the above receptors, which showed significant binding affinity with these receptors. In order to validate the in-silico findings, in-vitro evaluation of the test compound was also carried out. In combination, catharanthine reduced the minimum inhibitory concent...