Study on the optimum extracting conditions of total alkaloids and aconitine in Aconitum szechenyianum Gay

Objective: To optimize the conditions for extractions of total alkaloids and aconitine from Aconitum.szechenyianum Gay.Methods: Using L16(45)orthogonal test and univariant methods,the exrtaction conditions of total alkaloids aconitine in Aconitum szechenyianum Gay.were optimized under the guidance of the content determination of total alkaloids with bromophemol blue colorimetry and aconitine with HPLC method,respectively.Results: The obtained optimum condition of total alkaloids was that the material was refluxed in 6 times(m/v)acidic alcohol solution(alcohol: pH 3.0 HAc=85∶15,v/v)for 1 h with 3 times.The condition of aconitine was that the material was refluxed in 4 times(m/v) acidic alcohol solution(alcohol: pH 3.p HAc=15∶85,v/v)for 0.5 h with 3 times. Contents of total alkaloids and aconitine in Aconitum.szechenyianum Gay.were 0.980% and 0.109%,respectively.Conclusion: Through the test and verify,the optimum extraction methods of total alkaloids and aconitine are rational.