Transcriptomic analysis unravels the modulating mechanisms of the biomass and value-added bioproducts accumulation by light spectrum in Eustigmatos cf. Polyphem (Eustigmatophyceae).

Light spectrum can influence microalgal growth and metabolites accumulation significantly. However, the related mechanism has not been fully elucidated. Here, an oleaginous microalga Eustigmatos cf. polyphem, which also featured with high content of palmitoleic acid (POA) and β-carotene, was cultured with LEDs-based red light (RL) and blue light (BL). The results showed that the biomass, total lipid content and POA content were much higher under RL than these under BL, regardless of nitrogen concentration. However, the β-carotene content under RL was significantly lower than that under BL. Transcriptomic analysis revealed that photosynthesis, central carbon metabolism, fatty acid and glycerolipid biosynthesis were elevated, supporting the fast cell growth and high lipid content with POA under RL. In contrast, upregulation of key enzymes in carotenoids biosynthesis and suppression of β-carotene conversion promoted β-carotene accumulation under BL. These findings provide a feasible strategy for promoting lipids, POA and β-carotene in E. cf. polyphem.