Molecular Detection of Resistance Genes to Leaf Rust Lr34 And Lr37 in Wheat Germplasm

Abstract Leaf rust, caused by Puccinia triticina , is the most common rust disease of wheat. Using molecular markers technology, with a wide variety of approaches, can improve the efficiency of the selection strategies in wheat breeding programs. One of the most known resistance gene to leaf rust is Lr34 , involved in adult plant resistance (APR) or “slow rusting” type. In order to obtain cultivars with good levels of protection several resistant gene complexes need to be combined. Another gene that confers resistance to a wide range of races of Puccinia triticina , is Lr37 , originated from Triticum ventricosum , 2NS chromosome. Our study was focused on detection of the Lr34 and Lr37 genes in 50 wheat breeding lines using molecular markers. The molecular assay showed the presence of Lr34 resistance allele in 62% (homozygous genotypes) while the Lr37 resistance allele was detected in 40% of the total genotypes analysed. Also, we found one line that carries both resistance alleles.