Humoral and cell mediated immunomodulatory effect of Lactobacillus plantarum isolate from crop of guinea fowl in host species and broiler chicken

The study was aimed to investigate the effect of feed supplements viz: Lactobacillusplantarum LGFCP4 (laboratory isolate from crop of Guinea fowl), Lactobacillus acidophilus (NCDC, Karnal) and in-feed antibiotic bacitracin methylene disalicylate (BMD) on humoral and cellular immunity in guinea fowl and broiler chicken. During the entire experimental period, treatment groups were provided with different dietary treatments (T1-basal diet (Negative control), T2-Antibiotic growth promoter BMD 20g/100kg feed (Positive control), T3–1×108cfu of L.acidophilus/gm fermented feed +MOS 1g/kg feed and T4–1×108cfu of lab isolated L.plantarumLGFCP4/gm fermented feed+ MOS 1g/kg feed. On 21st day of post ND vaccination, the log2 RD-HI antibody titre in both species did not differed significantly (P>0.05) among different dietary groups even though highest titre was found in T4 group (6.12±.12) and lowest titre was found in T2 group (5.62±.32). Lymphocyte proliferation assay was used to estimate the cell mediated immune response to ND vaccine. Significantly higher mitogenic stimulation index in T-cell proliferation was observed in T4 group (1.57±.04) followed by T3 (1.33±.03). The results of the study showed that L. plantarum LGFCP4 can be applied as a species specific probiotic strain in guinea fowl and broiler chicken diet to augment immunity.