HPLC Method for Quantification of Five Compounds in a Parenteral Form Used in Treatment of Companion Animals.

This article describes a novel HPLC method able to quantify four active substances: tylosin tartrate, prednisolone, chloramphenicol and vitamin B12 and one excipient benzyl alcohol for the first time in <15 min. The analysis was conducted using a HyPurity Advance chromatographic column, a binary mobile phase which contains water (60%) and methanol (40%) as organic modifiers delivered isocratically at room temperature. The detection was established to be optimum at 260 nm. Limit of detection and limit of quantification were established experimentally, using the ratio signal-to-noise methodology. The method was validated in accordance with ICH and USP guidelines with respect to selectivity, linearity, limits of detection and quantitation, accuracy, precision and robustness and was applied on commercial available veterinary drug.