Testing the absorption of the extracts of Coreopsis tinctoria Nutt. in the intestinal canal in rats using an Ussing chamber.

Abstract Ethnopharmacological relevance Coreopsis tinctoria Nutt mainly distributed in Hetian region of Xinjiang at an altitude of 3000 m, which is used as Uyghur traditional medicine because of its clearing heat, promoting circulation and removing toxicity and antihypertension, ect. effect. Aim of the study This research was to study the four ingredients in the extracts of Coreopsis tinctoria Nutt. that are absorbed in different intestinal segments in rats to lay the foundation for further study on the effective constituents, tissue distribution, metabolism, and spectrum-effect relationships of these extracts. Materials and methods High, medium, and low concentrations were prepared according to their pharmacological effects. Quantitative analysis multi-components by single marker was used to test the cumulative absorption volume Q, absorption rate constant Ka, and apparent permeability coefficient P app of the four main ingredients in C. tinctoria Nutt. extract in different intestinal segments in rats using a Ussing chamber model and high-performance liquid chromatography. Results The P app of chlorogenic acid and flavanomarein in the duodenum, jejunum, ileum, and colon were 1.0×10 −6 to 10×10 −6  cm s −1 . P app of marein in the duodenum and jejunum was −6 , and was 1.0×10 −6 to 10×10 −6  cm s −1 in the ileum and colon. P app of 3,5- O -dicaffeoylquinic acid in the duodenum was −6  cm s −1 , while it was 1.0× 1 0 −6 to 10×10 −6  cm s −1 in the jejunum, ileum, and colon. Conclusions All four chemical components of the plant extract can be absorbed by the intestinal canal of rats, which conforms to zero-order absorption; the ileum presented the best absorption.