Determination of the formal reduction potential of Lumbricus terrestris hemoglobin using thin layer spectroelectrochemistry.

Abstract The formal reduction potential ( E ° ′ ) of Lumbricus terrestris hemoglobin was determined using thin layer spectroelectrochemistry as 0.073 (±0.005) V vs Ag/AgCl (0.281 V vs SHE, standard hydrogen electrode). Nernst plots of Lumbricus terrestris hemoglobin with tris-bipyridinecobalt(II) as a mediator titrant have similar linear slopes as Nernst plots of horse heart myoglobin with hexaamineruthenium(II) as a mediator titrant.