In vitro potential antimicrobial activities of medicinal plants, Cirsium arvense and Cirsium japonicum

Antimicrobial activities of Cirsium species (Cirsium arvense and cirsium japonicum) was evaluated against six bacterial species, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa (clinical strain/PIMS), Enterobacter (clinical strain/PIMS), Staphylococcus aureus (MRSA, clinical strain/PIMS) and six fungus Trichophyton longifusus, Candida albicans, Aspergillus flavus, Microsporum canis, Candida glaberata and Fusarium solani. The methanolic extracts of these plants were fractionated with n-hexane to get (F1), chloroform (F2), ethyl acetate(F3), nbutanol(F4) and methanolic fraction (F5). The agar well diffusion assay and agar dilution susceptibility testing were carried out to determine the zone of inhibitions and the minimum inhibitory concentration, respectively. Ager well diffusion methods was used for antifungal activity. The zones of inhibition were determined using standard agent. The entire fraction showed some activity against these pathogens, but F2 of both plants shows very good activity. The present study explains the potential antimicrobial properties of C. arvense and C. japonicum. From these observations, it is clear that Cirsium species contain antimicrobial constituents.