The effect of feeding live yeast cultures on ruminal pH and redox potential in dry cows as continuously measured by a new wireless device

Abstr Act : An experiment was carried out on four dry Holstein cows fitted with rumen cannulas that were divided into two groups. The crossover design experiment was divided into 4 periods of 3 weeks. Each period consisted of a 17-day preliminary period followed by a 4-day experimental period. Cows were fed twice daily the total mixed ration based on maize silage and concentrate. Control cows (Control) received the basal diets while experimental animals (Yeast) received the basal diet supplemented with 3.0 g of live yeast (BIOSAF Sc 47, Lesaffre, France) at each feeding. During each experimental period ruminal pH and redox potential (Eh) were monitored continuously using a developed wireless probe. Further, in each experimental period five samples of ruminal fluid were taken at 6:30, 8:30, 10:30, 13:30 and 16:30 h to determine the content of volatile fatty acids, lactic acids and ammonia. On the last day of each period, blood samples were taken for determina - tion of blood parameters and acid-base balance. Average daily dry matter intake throughout the experiment was 8.2 kg/day and was not affected by the treatment. The average ruminal pH in Control was 6.16 that was significantly lower than in Yeast, being 6.26 ( P 0.05). Lactate and ammonia concentrations at individual sampling times and overall mean did not differ significantly between treatments ( P > 0.05). Blood pH and CO 2 were not affected by the treatment.