First Report of Bloat Nematode (Ditylenchus dipsaci) Infecting Garlic in Ohio

Bloat nematode, Ditylenchus dipsaci (Kuhn) Filipjev (also known as stem and bulb nematode), is a key pest of garlic (Allium sativum) globally (1) as heavy infestations can lead to complete crop loss. Although not a major crop in Ohio, garlic is grown in diversified vegetable production systems. In July 2013, diseased garlic bulbs were received from a grower in Lorain County, OH, from a field with wide symptom distribution. Bulbs were discolored, exhibited splitting, and had basal plate damage including reduced roots. Nematodes were extracted for examination by placing bulb slices in water. Recovered nematodes had morphological characteristics of D. dipsaci, including a short stylet with prominent knobs, a distinct median esophageal bulb, a basal bulb slightly overlapping the intestine, a conical and pointed tail, and males with distinct bursa (1). To confirm the identity of the nematode, further morphological and molecular studies were performed. Nematode images were captured on a DM IRB inverted microsco...