Study on the extraction, denaturation, renaturation and purification of inclusion body-expressed bovine growth hormone.

The conditions of purification,denaturation and renaturation of recombinant Bovine growth hormone(BGH) were researched in order to get a good renaturation result.The fermentative thalluses were disrupted through high-pressure homogenization,and the inclusion body obtained by centrifugalization was denatured in 7.5 mol/L urea for 24 h,diluted with 100 mmolL Tris·HCl at pH 10.0 until the urea concentration was 4.5 molL,and renatured at 4℃ for 48 h.Dialysing 20 mmolL Tris·HCl at pH 9.0 and removing salt could successfully renature the recombinant BGH.The renatured recombinant BGH was concentrated with 100 K and 5 K ultrafilters and purified by fast protein liguid chromatography(FPLC),and thus the target protein of high purity could be got.