Genetic manipulation of Streptomyces species.

This unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR. Curr. Protoc. Microbiol. 19:10E.3.1-10E.3.26. © 2010 by John Wiley & Sons, Inc. Keywords: Streptomyces; protoplast; conjugation; genomic DNA isolation; transformation